Medical research technical roadmap
2025-03-06 10:39:09 154 1 Report 0
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This medical research technical roadmap outlines a comprehensive approach to understanding the role of LncRNA PVT1 and miR-149 in diabetic nephropathy. The roadmap includes studies on diabetic nephropathy in mice and patients, employing methodologies such as ELISA for inflammation markers, H&E staining for kidney injury, and real-time PCR for miR-149 expression. By integrating biological databases with experimental results, the research aims to elucidate the mechanisms and clinical significance of LncRNA PVT1 interactions. The roadmap culminates in drawing conclusions that could inform future therapeutic strategies for diabetic nephropathy.
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Outline/Content
NG
HG+siPVT1
ELISA Detection of Inflammation-Related Indicators
Health Group
Diabetic nephropathy in mice
Serum
H&E Staining for Detecting Kidney Injury
Study 2: The Mechanism of Action of LncRNA PVT1and mi-149 in Diabetic Nephropathy Mice
ELISA detection of BUN and Scr levels
Draw the conclusion of the paper
MPC5 cells
Targetscan Human prediction combined
Diabetic nephropathy patients
The biological database combined with experimental results predicts the binding of Lnc PVT1 and miR-149
Investigate the background of the topic and clarify the research significance.
Research Conclusions
ELISA detection of inflammation and oxidative stress markers
Western blot detection of miR-149
Diabeticnephropathyin mice
Research Methodology
Investigation of Lnc PVT1 and miR-149 Expression in Patients with Diabetic Nephropathy
PASM staining detects glomerular basement membrane
Kidney tissue
Real-time PCR detection of miR-149
Correlation between LncRNA PVT1 and mi-149
RNA Fluorescence In Situ Hybridization Technique
Real-time PCR detection of factor expression
Exploring the Clinical Research of LncRNA PVT1 in Diabetic Nephropathy
Correlation of LncRNA PVT1 with Indicators
Study Three: The Impact and Mechanism of LncRNA PVT1and mi-149 on Diabetic Nephropathy in Mice
HG
Research content
Study 1: Investigating the Expression of LncRNA PVT1and miR-149 in Patients with Diabetic Nephropathy
Dual-luciferase assay
Research Approach
Podocyte injury model
HG+siNC
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